Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 6th Global Summit on Aquaculture & Fisheries Osaka, Japan.

Day 1 :

Keynote Forum

Nchumbeni Humtsoe

Directorate of Fisheries, Government of Nagaland, India

Keynote: Status of fisheries development in Nagaland

Time : 10.25 am to 11.05 am

Conference Series Aquaculture Summit 2017 International Conference Keynote Speaker Nchumbeni Humtsoe photo
Biography:

Dr.Nchumbeni Humtsoe obtained her Ph.D. Degree from University of Mumbai, India on the topic  “Growth performance and biochemical composition of Labeo rohita to feed containing Katelysia opima and Nerita species,” and completed a course on “Professional planning and development programme in fisheries” from CIFE, Kolkatta, India . She had represented scientist team from India and attended 7th Indo-Pacific fish Conference at Taiwan and presented a paper on topic, “Effect of arsenic on the enzymes of freshwater fish Labeo Rohita” which was published in reputed international journal. She had presented research papers on several national conferences. She is a competent researcher and can work well in team with good communication skill and tackle any challenges positively. She is specialized in fish nutrition and feeding technologies. She is a life member of Indian Fisheries Association since 2008 and currently working as a Fishery Inspector in the Department of Fisheries, Government of Nagaland, India.

Abstract:

Nagaland is a vibrant hill state located in the extreme north eastern end of India. Nagaland has well fisheries potential. It is endowed with rich and unique natural resources which provide immense potential for aquaculture development. Fishery Department is responsible for fishery development in the state and central programmes. With the expansion of the Department and diversification of fisheries development activities in the state Department has subsidiary establishment in all the Districts and Sub-Divisions. With vast and varied fisheries resources, fishery plays an integral part which has tremendous potential of becoming a major contributor towards the improvement of states economy providing livelihood and employment to the local populace. Special efforts have been made to promote fish culture by way of adopting semi-intensive to intensive fish culture, paddy cum fish culture, reservoir fisheries, integrated farming etc. Fisheries Department in Nagaland focuses towards achieving a level of self-sustenance in fish production through identification of weaker aspects of the resource utilization and development such as managing the rivers and reservoirs ensuring responsible fishing and sustainable fisheries, efficient application of technologies and strengthening the domestic marketing system. The Department envisages local production to about 8605 MT and in addition a bulk quantity of 3800-3850 MT fish may be supplemented from outside the state to meet the demands of fish consumption. This available fish consumption could achieve a per capita consumption of 6.23kgs only which is much below the nutritional requirement of 11kgs per capita consumption as per WHO recommendation. Thus there exists a huge demand supply gap in the state as far as the production is concerned. The average productivity levels of fish envisage to achieve from ponds, paddy cum fish culture and reservoir fisheries is 2150, 355 - 500 and 158 kgs/ha/yr respectively. Doyang reservoir, the biggest reservoir in Nagaland having a total water area of 2258 hectares is one of the main fishery assets of the state. The depth of the reservoir is about 75-80m deep and area of catchment is about 26.6 km. Reservoir covers around 17 villages. At present there are around 350-400 fisherman depending on fishing for their livelihood at Doyang reservoir. In spite of the available resources aquaculture has not made much impact in the state so far. Immediate needs and adoption of better management practices could augment the present fish production in the state.

Keynote Forum

Nyan Taw

Senior Technical Advisor/General Manager, Blue Archipelago Berhad

Keynote: Biosecurity in Aquaculture Systems with Special Emphasis on Shrimp Farming

Time : 09:45 am to 10:25 am

Conference Series Aquaculture Summit 2017 International Conference Keynote Speaker Nyan Taw photo
Biography:

Nyan Taw received his Ph.D. from the University of Tasmania, Australia. He served as short term consultant for FAO and World Bank funded projects in Saudi Arabia and Vietnam. He was a technical counter-part for ADB and JICA projects to develop the fisheries sector in Myanmar from 1976 to 1987. In 1988, he joined the FAO of the UN and served in aquaculture projects in Indonesia, Vietnam and the Philippines culminating the position of CTA. From 1995, Dr Nyan served as Production Director at a number of locations in Indonesia. In 2002, he joined CP Indonesia, as VP where he initiated biofloc technology. Later he served as SVP for Dipasena Group, Indonesia. He served Blue Archipelago as GM and developed a biosecure, modular RAS system shrimp farm from 2009 to 2015 in Malaysia. He has provided consultancy for shrimp farming companies in South & Central America, Middle East and Asia He conducted Shrimp Biofloc Technology workshops for shrimp farmers in Malaysia, Australia, India, Saudi Arabia, Indonesia, Thailand & Myanmar. He co-authored a chapter in the book by Avnimelech on Biofloc Technology (2012 & 14).

Abstract:

According Lightner (2003) biosecurity is excluding pathogens from stock (i.e. post larvae and brood stock), especially through the use of quarantine and specific pathogen (SPF) certified stocks, and restricting imports of live and frozen shrimps. Excluding vectors and external sources of contamination and preventing internal cross contamination from hatcheries and farms. The first major outbreaks of shrimp disease (WSSV) was from late 1994 in Asia which was controlled until recently crossover to Mexico, Brazil and to Australia in early 2017. Another outbreak of shrimp disease EMS started from China in 2009 and spread to Vietnam, Malaysia and Thailand. In 2013 crossed over to Mexico. The revenue lost estimated by FAO due this EMS outbreak was around USD 1.2 Billion in one year. The outbreaks were believed to be due to lack in biosecurity. Recently due to major loses of shrimp farms at Gold Coast due to viral (WSSV) outbreaks (McElroy, 2017), Australian Government is aware of the potential biosecurity risk that could damage market access for their fisheries and aquaculture industry and significantly increase production costs for farmers (Oz Fisheries, 2017). Most locations in Asia are not viral free. One must be able to operate amid viral threats. One important factor is to understand the character of virus you are trying to protect from. Biosecurity starts with quality of farm design. This is followed by biosecure operation system using SPF Post Larvae. With quality farm design, operation system and biosecurity awareness training, the viral (WSSV) issue can be minimized. Farm biosecurity begins with design and construction of farm. Development of shrimp farm layouts from simple pond base flow through system during 1980s. At present with modular system by using reservoirs to treat incoming water provide biosecurity required to control the emerging viral issues (Nyan Taw, 2005, 2008 & 2011). The success or failure in shrimp aquaculture is how successful one can prevent and control the viral (mainly WSSV) out breaks. Biosecurity had been applied in Indonesia since introduction of L. vannamei from 2002 (Nyan Taw et al, 2002, 2007, 2008 & 2009; Nyan Taw, 2005 & 2010). Present lecture covered mainly on shrimp aquaculture biosecurity for sustainable production.

Keynote Forum

Nchumbeni Humtsoe

Directorate of Fisheries, Government of Nagaland, India

Keynote: Status of fisheries development in Nagaland
Conference Series Aquaculture Summit 2017 International Conference Keynote Speaker Nchumbeni Humtsoe photo
Biography:

Dr.Nchumbeni Humtsoe obtained her Ph.D. Degree from University of Mumbai, India on the topic  “Growth performance and biochemical composition of Labeo rohita to feed containing Katelysia opima and Nerita species,” and completed a course on “Professional planning and development programme in fisheries” from CIFE, Kolkatta, India . She had represented scientist team from India and attended 7th Indo-Pacific fish Conference at Taiwan and presented a paper on topic, “Effect of arsenic on the enzymes of freshwater fish Labeo Rohita” which was published in reputed international journal. She had presented research papers on several national conferences. She is a competent researcher and can work well in team with good communication skill and tackle any challenges positively. She is specialized in fish nutrition and feeding technologies. She is a life member of Indian Fisheries Association since 2008 and currently working as a Fishery Inspector in the Department of Fisheries, Government of Nagaland, India.

Abstract:

Nagaland is a vibrant hill state located in the extreme north eastern end of India. Nagaland has well fisheries potential. It is endowed with rich and unique natural resources which provide immense potential for aquaculture development. Fishery Department is responsible for fishery development in the state and central programmes. With the expansion of the Department and diversification of fisheries development activities in the state Department has subsidiary establishment in all the Districts and Sub-Divisions. With vast and varied fisheries resources, fishery plays an integral part which has tremendous potential of becoming a major contributor towards the improvement of states economy providing livelihood and employment to the local populace. Special efforts have been made to promote fish culture by way of adopting semi-intensive to intensive fish culture, paddy cum fish culture, reservoir fisheries, integrated farming etc. Fisheries Department in Nagaland focuses towards achieving a level of self-sustenance in fish production through identification of weaker aspects of the resource utilization and development such as managing the rivers and reservoirs ensuring responsible fishing and sustainable fisheries, efficient application of technologies and strengthening the domestic marketing system. The Department envisages local production to about 8605 MT and in addition a bulk quantity of 3800-3850 MT fish may be supplemented from outside the state to meet the demands of fish consumption. This available fish consumption could achieve a per capita consumption of 6.23kgs only which is much below the nutritional requirement of 11kgs per capita consumption as per WHO recommendation. Thus there exists a huge demand supply gap in the state as far as the production is concerned. The average productivity levels of fish envisage to achieve from ponds, paddy cum fish culture and reservoir fisheries is 2150, 355 - 500 and 158 kgs/ha/yr respectively. Doyang reservoir, the biggest reservoir in Nagaland having a total water area of 2258 hectares is one of the main fishery assets of the state. The depth of the reservoir is about 75-80m deep and area of catchment is about 26.6 km. Reservoir covers around 17 villages. At present there are around 350-400 fisherman depending on fishing for their livelihood at Doyang reservoir. In spite of the available resources aquaculture has not made much impact in the state so far. Immediate needs and adoption of better management practices could augment the present fish production in the state.

Keynote Forum

W.M.T.B.Wanninayake

Wayamba University of Sri Lanka, Sri Lanka

Keynote: Maintaining the balance between aquatic ecosystems and fishing activities
Conference Series Aquaculture Summit 2017 International Conference Keynote Speaker  W.M.T.B.Wanninayake photo
Biography:

W M T B Wanninayake (Senior Marine Biologist) formerly served as Professor of Fisheries and Aquaculture, Wayamba University of Sri Lanka and Chairman, Ocean University of Sri Lanka. He is a former Senior Researcher of the National Aquatic Resources Research and Development Agency (NARA), Colombo. He earned his BSc in Zoology and MPhil in Aquaculture (Crustacean Research) from the University of Kalaniya, Sri Lanka and PhD in Bivalve Research from the University of Liverpool, United Kingdom. He contributed immensely in developing the prawn farming industry and hatchery sector in Sri Lanka. He has been recognized for introducing oysters, mussels, clams and cockles farming in Sri Lanka and is also a pioneer in introducing pearl culture in freshwater reservoirs. He is a Founder Member of the Asian Fisheries Forum (AFS) and Sri Lanka Association for Fisheries and Aquatic Resources (SLAFAR). He has wide experience and a profound understanding of aquaculture and aquatic environment in many European and Asian countries. Currently, he serves in advisory capacity to few ministries related to his work in Sri Lanka.

Abstract:

A degradation of ecosystems is a significant direct effect of both fishing and aquaculture activities. The aquatic environment provides humanity with a large number of services ranging from food security and climate regulation to nutrient cycling and storm protection. Ecological services are classified into physical structure services, biotic services, biogeochemical services, information services, and social/cultural services. For the past few decades, these services have supported lives and livelihoods in many sectors but overfishing, pollution, habitat destruction, ocean warming, destructive aquaculture practices and acidification have threatened ecosystem services. Even the recent trends of using many marine and freshwater habitats of Sri Lanka for various goods and services has created many challenges similar to other parts of the world. The fundamental challenge is to identify options and opportunities to integrate the production of commodities together with the preservation of ecological values. Presently users compete for sharing the benefits derived from the aquatic ecosystems with little or no incentive to protect or improve those goods and services for future generations. As a result, aquatic ecosystems are facing increased risks towards enhancing their prospects of future wealth, livelihoods, and food security. It is, therefore, important to reflect at this juncture how we can maintain the balance between aquatic ecosystems and so called fishing activities. At present we have technology and information integrating the world more than any other time in history, and this has paved way to solve many problems. The current understanding of the relationships between ecological services and functional groups of species, and biological communities of aquatic ecosystems vary in different regions of the world. Nevertheless, the consequences of human impacts on aquatic habitats are also discussed, including loss of resilience, or buffer capacity. Such loss may impair the capacity to successfully recover aquatic ecosystems, and as a consequence the quality and quantity of their delivery of ecological goods and services have diminished. We need to improve the stewardship of aquatic habitats. Increasing public awareness can easily change the global scale consumer behaviors and choices. These challenges need to be addressed by global, public-private partnerships aiming at maintaining a sustainable balance among aquatic ecosystems, fishing, aquaculture and human wellbeing.

  • Advances in Aquaculture Nutrition
Speaker
Biography:

Bilikis Iyabo Uneke holds a Ph.D in Fisheries Biology. She is a leading researcher and lecturer in the Department of Biological Sciences, Ebonyi State University, Nigeria. In the last ten years, her work as focused on aquaculture, stock assessment, modeling, fisheries management and policy. She has worked extensively on the food, feeding habits and nutrient requirements of teleost fishes of the inland waters of southeastern Nigeria

Abstract:

The requirement of African catfish, Heterobranchus longifilis for dietary was determined. Two hundred and seventy fingerlings samples of H. longifilis (mean weight 2.3±0.1g) were stocked in eighteen (18) plastic bowls, each of 25 litres capacity at a density of 15 fish samples per bowl. Zinc sulphate of 0.1%, 0.5%, 1.0%, 1.5%, 2.0% and 0.0% were assigned randomly to the water baths which were designated as D1, D2, D3, D4, D5 and D6 respectively and each with three replicates. The fish samples were fed at 5% body weight per day thrice daily at 8:00hrs, 12:00hrs and 16hrs. Water in experimental bowls was changed weekly. Fish weights were taken fortnightly and feed were adjusted (to maintain it at a constant 5% of the body weight of fish). The result showed that the performance of fish diets with different levels of zinc sulphate was significantly different (P = 0.05) between treatments. Fish fed diet 1.5% of zinc sulphate inclusion level performed significantly better than the ones fed with the other diets. Fish fed with 2.0% zinc sulphate inclusion level showed high Food Conversion Ratio (FCR) indicating that when the optimum dietary requirement of zinc is exceeded, it can adversely affect the feed conversion ability of the fish, resulting in poor weight gain. Hence, zinc should be included in the diet of fish at the required level to avoid adverse effect which could be harmful not only to the organism but to tertiary consumer (Human beings).  

Speaker
Biography:

Mahmoud Hafezieh has his expertise in Aquaculture technology on fish and shellfish nutrition and their live food. He is  working Iranian  fisheries Sciences research institute Also he is as a scientific staff of AZAD university in Tehran and during his 23 years work,  he published more than 40 Scientific articles base on his research projects, Also published 7 Books specifically live food and inert food in aquaculture. recently he focused on seaweed as ingredients in fish and shrimp food which this article is a part of one.

Abstract:

Statement of the Problem: Shrimp food factory in Iran used high amounts of chemical binders which is not environment friendly.

The purpose of this study is binding effect of Sargassum ilicifolium on  Litopenaeus vannamei pellets.

Methodology & Theoretical Orientation: Shrimp feed bound with a brown seaweed, Sargassum ilicifolium powder replaced at three level with protein resources in laboratory. Treatments were incorporated at zero (used 2% formaldohyde sorbate=FS),  5, 10 and 15% level of seaweed while in the commercial feed(CF) pellet which used a chemical binders routine in shrimp feed factory. After 30 minutes of exposure to sea water (equal to the Oman Sea water, 33 ppt salinity).

Findings: 15%  treatment maintained a 25% pellets afloat while zero treatment had the least. There was a difference in the percentage number of pellets afloat between 5 minutes and 30 minutes of exposure to water (P<0.05). There was also a difference in the water stability of the different levels seaweed used (P<0.05).

Conclusion & Significance: 15% treatment had the highest water stability (57.70±1.60) after 50 minutes of exposure to water.

Mahmoud Hafezieh

Iranian Fisheries Sciences Research Institute(IFSRI), Agricultural Research, Education & extension Organization- Iran

Title: Water Stability and Floatation Test of Shrimp (Litopenaeus vannameii) Pellets Using Brown Seaweed Powder (Sargassum ilicifolium)
Speaker
Biography:

Mahmoud Hafezieh has his expertise in Aquaculture technology on fish and shellfish nutrition and their live food. He is working Iranian  fisheries Sciences research institute Also he is as a scientific staff of AZAD university in Tehran and during his 23 years work,  he published more than 40 Scientific articles base on his research projects, Also published 7 Books specifically live food and inert food in aquaculture. recently he focused on seaweed as ingredients in fish and shrimp food which this article is a part of one.

Abstract:

Statement of the Problem: Shrimp food factory in Iran used high amounts of chemical binders which is not environment friendly.

The purpose of this study is binding effect of Sargassum ilicifolium on Litopenaeus vannamei pellets.

Methodology & Theoretical Orientation: Shrimp feed bound with a brown seaweed, Sargassum ilicifolium powder replaced at three level with protein resources in laboratory. Treatments were incorporated at zero (used 2% formaldehyde sorbate=FS), 5, 10 and 15% level of seaweed while in the commercial feed(CF) pellet which used a chemical binders routine in shrimp feed factory. After 30 minutes of exposure to sea water (equal to the Oman Sea water, 33 ppt salinity).Findings: 15% treatment maintained a 25% pellets afloat while zero treatment had the least. There was a difference in the percentage number of pellets afloat between 5 minutes and 30 minutes of exposure to water (P<0.05). There was also a difference in the water stability of the different levels seaweed used (P<0.05).

Conclusion & Significance: 15% treatment had the highest water stability (57.70±1.60) after 50 minutes of exposure to water.

Speaker
Biography:

Dr.Nchumbeni Humtsoe obtained her Ph.D. Degree from University of Mumbai, India on the topic  “Growth performance and biochemical composition of Labeo rohita to feed containing Katelysia opima and Nerita species,” and completed a course on “Professional planning and development programme in fisheries” from CIFE, Kolkatta, India . She had represented scientist team from India and attended 7th Indo-Pacific fish Conference at Taiwan and presented a paper on topic, “Effect of arsenic on the enzymes of freshwater fish Labeo Rohita” which was published in reputed international journal. She had presented research papers on several national conferences. She is a competent researcher and can work well in team with good communication skill and tackle any challenges positively. She is specialized in fish nutrition and feeding technologies. She is a life member of Indian Fisheries Association since 2008 and currently working as a Fishery Inspector in the Department of Fisheries, Government of Nagaland, India.

Abstract:

A six months feeding experiment was conducted to evaluate the incorporation of five feed ingredients with the flesh of Nerita species in the diet of Labeo rohita. Fingerlings of Labeo rohita weighing 2-4 gm were divided into six groups and fed with prepared feeds using five different feed ingredients such as ground nut oil cake, tapioca flour, rice bran and wheat flour along with different levels of the flesh of Nerita species as experimental diets. A control group of rohus fingerlings was maintained on pelleted feed without adding the flesh of Nerita species. Fishes were fed twice daily with the respective test diets at the rate of 4% body weight during entire culture period of three months. Results regarding growth performance of L.rohita fingerlings fed on different experimental diets showed that the best growth performance of fish in terms of percentage live weight gain was noted as 131.68 % where as 46.60 % was noted with control diet which is prepared without adding the flesh of Nerita species . Maximum increase in length (2.90cm) was noticed in the fingerlings fed with highest level of the flesh of Nerita species compared to minimum increase in length (1.33 cm). Specific Growth Rate (SGR) per day of fish fed on different experimental diets was noted in the range of 0.22 - 0.61 percentage compared to control diet which was noted as 0.28 percentage. The feed conversion ratio observed 4.417 was highest in fish fed with highest level of the flesh of Nerita species compared to fish fed on control diet which is noted as 4.032. This study reveals that incorporation of the flesh of Nerita species  in different feed ingredients  is one of the best animal proteins in terms of growth and feed conversion which is optimum for growth of Labeo rohita.

Speaker
Biography:

A.T. Ramachandra Naik is Associate Professor, College of Fisheries, Mangalore 575002, Karnataka Veterinary, Animal and Fisheries Sciences University, India.

Abstract:

The freshwater prawn, Macrobrachium rosenbergii is a more popular crustacean cultured widely in monoculture system in India. It has got high nutritional value in the human diet. Hence, understanding its enzymatic and body composition is important in order to judge its flesh quality. Fish oil specially derived from Indian oil sardine is a good source of highly unsaturated fatty acid and lipid source in fish/prawn diet. A 35% crude protein diet with graded levels of Sardine oil as a source of fat was incorporated at four levels viz, 2.07, 4.07, 6.07 and 8.07% maintaining a total lipid level of feed at 8.11, 10.24, 12.28 and 14.33% respectively. Diet without sardine oil (6.05% total lipid) was served as basal treatment. The giant freshwater prawn, Macrobrachium rosenbergii was used as test animal and the experiment was lost for 112 days. Significantly higher gain in weight of prawn was recorded in the treatment with 6.07% sardine oil incorporation followed by higher specific growth rate, food conversion rate and protein efficiency ratio. The 8.07% sardine oil diet produced the highest RNA:DNA ratio in the prawn muscle. Digestive enzyme analyses in the digestive tract and mid-gut gland showed the greatest activity in prawns fed the 8.07% diet.

Speaker
Biography:

Len S. Smith holds the position as Chief Business Officer of Heliae. Heliae is an applied microalgae research, development, products and technology provider based in Arizona, USA. Mr. Smith has been with Heliae since 2014. Prior to his current role at Heliae, he held management and leadership positions with leading biotechnology and pharmaceutical companies in the USA.  He holds degrees in biology, environmental science, and business from leading US universities.

Abstract:

Omega-3 fatty acids, such as DHA and EPA, have been demonstrated to have critical roles in both fish and human nutrition.  Increasingly, fish products are sourced from farmed fish.  However, many farmed fish have been demonstrated to lack the omega-3 fatty acid content of typical wild fish counterparts.  Moreover, in a period where consumers increasingly wish to obtain healthy dietary components, such as DHA, from fish, as compared to from dietary supplements, the amount of DHA in fish has reportedly fallen.  Most omega-3 sources used in feed today are not efficient at achieving optimal DHA:EPA ratios as microalgal sources, as these traditionally used sources usually have relatively high amounts of EPA to DHA.  DHA-rich microalgae can provide an alternative tool for feed formulation, one that allows feed formulators a way to supply precise amounts of DHA into the feed.  In addition, the DHA in microalgae DHA is naturally encapsulated, providing a more stable source of DHA.  Heliae has sourced a novel DHA-rich microalgae and developed a production technology that will allow this product to be widely available to the aquaculture industry.

  • Diseases in Aquaculture

Session Introduction

Nyan Taw

Sustainable Shrimp Farming in Biosecure RAS and Biofloc Technology: Commercial Experience and Approaches to Disease Control-Malaysia

Title: Former Chief Technical Advisor & Consultant for FAO of the UN and Consultant for World Bank Projects, Malaysia.
Speaker
Biography:

Dr Nyan Taw received his Ph.D. from the University of Tasmania, Australia. He served as short term consultant for FAO and World Bank funded projects in Saudi Arabia and Vietnam. He was a technical counter-part for ADB and JICA projects to develop the fisheries sector in Myanmar from 1976 to 1987. In 1988, he joined the FAO of the UN and served in aquaculture projects in Indonesia, Vietnam and the Philippines culminating the position of CTA. From 1995, Dr Nyan served as Production Director at several locations in Indonesia. In 2002, he joined CP Indonesia, as VP where he initiated bio-floc technology. Later he served as SVP for Dipasena Group, Indonesia. He served Blue Archipelago as GM and developed a bio-secure, modular RAS system shrimp farm from 2009 to 2015 in Malaysia. He has provided consultancy for shrimp farming companies in South & Central America, Middle East and Asia He conducted Shrimp Biofloc Technology workshops for shrimp farmers in Malaysia, Australia, India, Saudi Arabia, Indonesia, Thailand & Myanmar. He co-authored a chapter in the book by Avnimelech on Biofloc Technology (2012 & 14).

Abstract:

Biosecure Biofloc technology applied in shrimp farming is adapted from the basic minimum water exchange shrimp intensive culture system used in Indonesia since early late 1990s. The system then was to position aerators within culture ponds to concentrate waste (sludge) into centre of ponds which were then siphon out physically or through central drain system. The aerators were operated almost 24 hours to have optimum culture pond environmental condition. The system creates clean water column and bottom feeding area and separated the sludge area (Taw, 2005; 2015, Taw, et al 2007). Shrimp bio-floc system is somewhat an upgraded system by introducing carbon such as molasses and wheat flour to develop a heterotrophic environment with zero water exchange which suspend bio-floc colony within pond water column. The bio-floc system is developed by developing algae first and later cross over to bio-floc to have self-nitrification process sets in (Taw 2014). Main economic benefits of shrimp bio-floc system are – better biosecurity, low FCR, higher production, higher energy efficiency and sustainable production. Biofloc system alone cannot prevent ever emerging shrimp diseases. However, bio-floc and bio-secure shrimp farm design, construction and operation system have shown to prevent diseases entering the farm facilities (Taw 2005; Taw et al 2008, & Taw & Setio 2014).  In Malaysia bio-secure modular RAS system with bio-floc technology has been applied at Blue Archipelago shrimp farm since October 2011 and has been operating successfully without any incident of EMS/AHPND which was a major threat to China, Vietnam, Malaysia and Thailand (Taw, et al. 2013 & Taw 2014).  According to In-Kwon (2012 &2014) there were more than 2,000 bacterial species in well-developed bio-floc water. This bio-floc may enhance immune activity based on mRNA expression of six immune-related genes – ProPO1, ProPO2, PPAE, ran, mas and SP1. A study at Bogor University, Indonesia and Ghent University, Belgium revealed that bio-floc system contributes to the enhancement of immune response and survival after IMNV challenge regardless the carbon source. The application of BFT brings about beneficial effect in disease control and management in shrimp culture (Ekasari, et al., 2014).

 

Nyan Taw

Former Chief Technical Advisor & Consultant for FAO of the UN and Consultant for World Bank Projects, Malaysia.

Title: Sustainable Shrimp Farming in Biosecure RAS and Biofloc Technology: Commercial Experience and Approaches to Disease Control-Malaysia
Speaker
Biography:

Dr Nyan Taw received his Ph.D. from the University of Tasmania, Australia. He served as short term consultant for FAO and World Bank funded projects in Saudi Arabia and Vietnam. He was a technical counter-part for ADB and JICA projects to develop the fisheries sector in Myanmar from 1976 to 1987. In 1988, he joined the FAO of the UN and served in aquaculture projects in Indonesia, Vietnam and the Philippines culminating the position of CTA. From 1995, Dr Nyan served as Production Director at several locations in Indonesia. In 2002, he joined CP Indonesia, as VP where he initiated bio-floc technology. Later he served as SVP for Dipasena Group, Indonesia. He served Blue Archipelago as GM and developed a bio-secure, modular RAS system shrimp farm from 2009 to 2015 in Malaysia. He has provided consultancy for shrimp farming companies in South & Central America, Middle East and Asia He conducted Shrimp Biofloc Technology workshops for shrimp farmers in Malaysia, Australia, India, Saudi Arabia, Indonesia, Thailand & Myanmar. He co-authored a chapter in the book by Avnimelech on Biofloc Technology (2012 & 14).

Abstract:

Biosecure Biofloc technology applied in shrimp farming is adapted from the basic minimum water exchange shrimp intensive culture system used in Indonesia since early late 1990s. The system then was to position aerators within culture ponds to concentrate waste (sludge) into centre of ponds which were then siphon out physically or through central drain system. The aerators were operated almost 24 hours to have optimum culture pond environmental condition. The system creates clean water column and bottom feeding area and separated the sludge area (Taw, 2005; 2015, Taw, et al 2007). Shrimp bio-floc system is somewhat an upgraded system by introducing carbon such as molasses and wheat flour to develop a heterotrophic environment with zero water exchange which suspend bio-floc colony within pond water column. The bio-floc system is developed by developing algae first and later cross over to bio-floc to have self-nitrification process sets in (Taw 2014). Main economic benefits of shrimp bio-floc system are – better biosecurity, low FCR, higher production, higher energy efficiency and sustainable production. Biofloc system alone cannot prevent ever emerging shrimp diseases. However, bio-floc and bio-secure shrimp farm design, construction and operation system have shown to prevent diseases entering the farm facilities (Taw 2005; Taw et al 2008, & Taw & Setio 2014).  In Malaysia bio-secure modular RAS system with bio-floc technology has been applied at Blue Archipelago shrimp farm since October 2011 and has been operating successfully without any incident of EMS/AHPND which was a major threat to China, Vietnam, Malaysia and Thailand (Taw, et al. 2013 & Taw 2014).  According to In-Kwon (2012 &2014) there were more than 2,000 bacterial species in well-developed bio-floc water. This bio-floc may enhance immune activity based on mRNA expression of six immune-related genes – ProPO1, ProPO2, PPAE, ran, mas and SP1. A study at Bogor University, Indonesia and Ghent University, Belgium revealed that bio-floc system contributes to the enhancement of immune response and survival after IMNV challenge regardless the carbon source. The application of BFT brings about beneficial effect in disease control and management in shrimp culture (Ekasari, et al., 2014).

  • Aquaculture Economics
Speaker
Biography:

José L. Fernández Sánchez is economist and professor of business economics and organization in the department of business administration at University of Cantabria (UC) in Santander (Spain). He also participates in the UC’s research group IDES. He gets a doctoral degree in Business Administration from University of Cantabria and two master degrees in Marketing (ESIC) and Economics (Queens College, CUNY). His research interests are related to corporate social responsibility, corporate reputation and strategy, as well as the sustainability of the primary sector and the food industry.

Abstract:

The purpose of this research is to analyze the effect that the market structure could have on the profit margins of the European (EU28) fish and seafood producers. This research employs the Structure–Conduct–Performance (SCP) paradigm to investigate the simultaneous relationship between price-cost margin (PCM), industrial concentration (IC), and technical efficiency (TE) in the European fish and seafood production industry. So, following Setiawan et al. (2013), the following non-recursive structural model can be formulated:

 

PCM = f (IC, TE, SIZE, GROWTH)

IC = g (PCM, GROWTH, CI)

TE = h (IC, SIZE)

 

This simultaneous system of equations has been estimated using the Full Information Maximum Likelihood (FIML) estimation method. Annual industry-macro data from different private and public sources (AMADEUS, STECF, and EUMOFA databases) for each of the EU28 countries during the period 2008–2013 has been employed to estimate the structural model parameters for the fishing and aquaculture sectors.

The findings about our structural model are presented in Table 1. So, a good fit of the whole model was obtained and estimation results are majorly in agreement with economic theory. The results show that market growth is negatively related to market concentration whereas capital intensity and the price-cost margin are positively related with industry concentration. Also, it has been found that higher industrial concentration leads to lower technical efficiency. On the other hand, technical efficiency is affected positively by the sector size (significance at the 1% level). Further, technical efficiency affects price-cost margin positively since technical efficiency lowers the per unit cost of production. Finally, industry concentration, technical efficiency, and market growth affect positively industry price-cost margins (at least, in the case of fishing sector, this effect has been statistically very significant) whereas industry size had a negative effect on the fish and seafood producers’ profit margins.

  • Aquaculture Technology and Engineering Applications
Speaker
Biography:

Dr. C.A. Saclauso is a Professor and Dean of the College of Fisheries and Ocean Sciences of the University of the Philippines Visayas, Miagao, Iloilo, Philippines. His interest is on trophic ecology of fish particularly, the interaction of feeding and social behavior with growth. His early work on cage culture of milkfish in marine waters provided opportunity in addressing stock density-dependent growth leading to the concept of bottom feeding. This paved the way for the development of the hydro-kinetic bottom feeder that improves feed access of fish in high density culture.  Dr. J.D. Toledo was formerly the Chief of SEAFDEC Aquaculture Department in the Philippines. He is presently the Technical Director of Feedmix Specialists Inc II at Pulilan, Bulacan, Philippines. His interest is on the reproductive biology of fish and in improving efficiency of operations for sustainable and profitable aquaculture production.

Abstract:

High density stocking often results in slow growth and low survival in aquaculture. This is attributed to diminished space, inequitable access to food, and aggressive behavior as a consequence of crowding and deterioration of water quality at high population densities. Under crowded conditions, aggression depresses growth that results in the deaths of subordinate conspecifics. In non-aggressive species like milkfish, crowding significantly affects size variance hypothesized to have been caused by reduced food access in high density culture where fish are fed from the top.  

The study aims to improve yield and profitability of milkfish production in high density culture through bottom feeding. With increased density, yield per unit volume is also expected to increase thus diminish the area required in generating equivalent production at lower stock densities.  

Milkfish at densities of 50 pieces m-3, 75 pieces m-3 and 100 pieces m-3 were reared for 120 days in replicated 5mx5mx5m floating net cages in a marine cove with a depth of 10 m and a flow rate of 8 cm sec -1. Fish were fed floating pellets through top demand feeder and hydro-kinetic bottom feeder.

Bottom feeding increased production of milkfish cultured at very high density.  Net yield (kg m-3) increased by 75% with the doubling of stocking density from 50 m-3 to 100 m-3. Size variance was reduced and condition index (K) and feed conversion ratio (FCR) were improved. Bottom feeding optimizes food utilization through equitable and better access to food hence, reduces feed wastage. Bottom feeding and high density stocking enhances production efficiency without compromising environmental integrity.

Speaker
Biography:

Mr. Muhammad Usman, Former Director General of Agricultural Research System, Government of Pakistan who retired from service after a spotless career of about 35 years with senior level experience on research and development of integrated agricultural industries with regard to livestock and dairy development, poultry, aquaculture and fishery, apiculture, sustainable agricultural production system, fruits and vegetable, seed production, bioenergy and other cash crops.Mr. Usman is basically an agricultural scientist with specialization of agricultural/ chemistry working as plant breeder with regard to the yield and quality of various agricultural crops, released several varieties, presented and published research papers on crops and renewable energy in the different conferences like Geneva.Mr. Usman established “Prominent Agro Based Industries SDN BHD” in Malaysia and “Foundation for Rural Development in Pakistan”, with primarily aims to work on integrated agricultural project for Rural Development through improvement in agriculture for rehabilitation of affected area.

Abstract:

The aims of presentation consist of aquaculture, fishery and poverty were studied to find out the role of aquaculture and fishery industries on the poverty alleviation of South Asia like Pakistan. Aquaculture means the fish or shellfish farming-refers to breading, rearing and harvesting of plants and animals in all types of water environments including ponds, river, lakes and the ocean. Researcher and aquaculture producers are "farming" all kinds of freshwater and marine species of fish, shellfish, and plants.  Aquaculture consists of two types. Marin Aquaculture refers to the culturing species that lives in the ocean and consist of sea ranching, surface lines, sub-surface lines, rack, sea cages, pond and hatcheries. Freshwater aquaculture is the culture of aquatic species within and dependent on fresh water environment. The objective of the aquaculture is the production of protein rich, nutritive, palatable and easily digestible human food at reasonable cost, production of sport fish, baitfish, ornamental fish, industrial fish. Research work conducted showed that showed that 85% fish productions are in sea marine and only 5% are in water growers commercially. The production of the different fish like sport, bait, ornamental, industrial, black tilapia, red tilapia, hybrid tilapia, black prawn, rid prawn are good food items and plays an important role in nutrition and health of aged people. Aquaculture provides protein rich food which are more efficient than other animals. It is concluded that improving aquaculture and fishery is the most powerful tool for absorbing millions of technical and non-technical people, increase income, create employment, the cheapest source of quality food which will consequently reduce poverty and without it life is almost impossible.

N. R. Chatterjee

West Bengal University of Animal & Fishery Sciences, Kolkata, India

Title: Title: Technology Development and its Dissemination in Aquaculture Sector
Speaker
Biography:

Prof. Chatterjee developed various innovative technology in Aquaculture and published more than 50 papers in reputed international journals. In 2015 he was awarded Honorary fellow by the Singapore Institute of Aquaculture. His book on “Induced Fish Breeding “published in 25th Oct. by Elsevier. Prof. Chatterjee is member and advisor of many academic body at national and international level.

Abstract:

World aquaculture production continues to grow, albeit at a slowing rate. According to the latest available statistics collected globally by FAO, world aquaculture production attained another all-time high of 90.4 million tonnes (live weight equivalent) in 2012 (US$144.4 billion), including 66.6 million tonnes of food fish (US$137.7 billion) and 23.8 million tonnes of aquatic algae (mostly seaweeds, US$6.4 billion). In this context, India is the second largest producer of fish and the second largest producer of fresh water fish in the world. Fishery plays an important role in contribution of rural economy and nutritional security. It has the highest contribution in agricultural GDP among all the allied fields of agriculture. But there is a severe concern gets highlighted when the per-capita fish availability is compared with its recommended level and production level is compared with the demand. A sharp gap can be identified, especially in case of aquaculture production which demands technological interventions to meet up this gap. The latest technological developments in fisheries sector offers better prospects for all sections of rural people particularly for those who have poor land base and abundant labour force. Despite significant advancement of fisheries sector there is still moderate to low adoption of modern fisheries production technologies.  There are considerable evidences which suggest that non-adoption of recommended technologies is often related to non-technological factors, such as social, psychological, cultural and economic factors. These barriers can only be solved by successful implementation of different fisheries development programmes. Several studies indicated that lack of adequate planning and continual evaluation is a major reason for the failure of the development of projects and extension activities (FAO,1996). Therefore, development of sustainable technologies for Aquaculture practices and effective extension communication strategies are needed. In recent years, there has been growing dissatisfaction with the poor rates of adoption of aquaculture technologies in resource-poor farming systems.  This poor adoption has resulted partly because when aquaculture technologies are developed, there is little input from farmers.  Participatory Technology Development (PTD) offers a way forward, through active, decision-making involvement of farmers in every stage of technology development. When new technology is being developed, researchers usually control all steps of the process. The Participatory Technology Development (PTD) approach involves farmer’s right from the beginning, when they are asked to identify their problems.

  • Biotechnology and Genetics in Aquaculture
Speaker
Biography:

Isagani P. Angeles Jr, Ph.D. is a registered Fisheries Technologist in the Philippines and a Professor/Director in the Provincial Institute of Fisheries (PIF), Isabela State University, Isabela, Philippines. He obtained his Ph.D. in Aquaculture in the Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan in 2015. He is the Project Leader in several research studies on the Development of Aqua Feed Diet and Alternative Technology on Tilapia and Prawn Production funded by ISU and Department of Science and Technology. His expertise encompasses the field of aquaculture management, particularly on feed and nutrition and fish/shellfish immunology.

Abstract:

The study evaluated the growth, haematology, glucose and  resistance to ammonia stress of Nile tilapia Oreochromis niloticus fed diets supplemented with different antioxidants. The fish were fed diet containing 80 mg kg-1astaxanthin (AX80), 50 g kg-1 malunggay Moringa oleifera (MO50) and 5 g kg-1 nutrafito plus (NP5) or basal diet (C) for 42 d. Weight sampling was conducted every 14 d while survival was monitored daily. Blood was collected to determine the haematological parameters and blood glucose level of fish before and after ammonia stress test. Wf, WG and SGR of NP5-fish were significantly higher than C-fish but comparable to AX80- and MO50-fish. However, other parameters such as PER and FCR were not significantly different among all treatments. There were significant results on some haematological parameters, Gluc level and survival before and after ammonia stress. Before ammonia stress, RBC of NP5-fish was significantly higher among treatments while HCT of NP5-fish was significantly higher than MO50-fish. After ammonia stress, HGB of AX80-fish was significantly higher than C-fish but comparable to MO50- and NP5-fish. On the other hand, RBC of NP5-fish was significantly higher than MO50- and C-fish. Moreover, HCT of NP5-fish was significantly higher than C-fish. Gluc level of NP5 was the lowest among treatments. In addition, AX80- and NP5-fish showed significantly higher survival than to the C-fish. These results indicated that fish fed diet supplemented with Nutra fit plus improved growth performance, stabilize some haematological parameters and glucose level and improved survival of O. niloticus exposed to ammonia stress.

Sadiqul Awal

School of Food, Plant and Animal Industries, Melbourne Polytechnic, Victoria Australia

Title: Reclamation of salt affected soils and production of microalgae biomass for commercial purposes.
Speaker
Biography:

Sadiqul Awal has completed his PhD at the age of 40 years from Deakin University Victoria Australia. He is currently working as a lecturer at Melbourne Polytechnic, Victoria Australia. He has published more than 20 papers in reputed journals and has been serving as reviewer in many journals. His research interest in aquaculture in inland ground saline water, microalgae, and estuarine environmental management.

Abstract:

 

The issue of the salinization of Australia’s inland ground water is a recurring and major environmental problem that is often the result of previous uses of the land for agricultural purposes and the use of certain irrigation practices. Inundation of land by saline water for long periods leads to its percolation into the surrounding soils, resulting in altered soil chemistry. Prolonged inundation inhibits the fixation of free nitrogen and halts mineralization, thus impairing soil fertility within a few years. The elevation of a water table that features increased salinity levels (sometimes approaching or even exceeding that of seawater) means that while the environmental ramifications are potentially massive (and include crop damage), it is true also that such waters are an untapped and largely unexplored aquatic resource. It is no doubt true that such inland areas present a wonderful opportunity to farm a variety of fish, molluscs and crustaceans (an opportunity that has thus far been realised on a relatively very small scale), but there is no doubt considerable scope to grow microalgae, which underpin the bottom of the food chain in aquatic systems and have an enormous number of potential uses. Microalgae are potentially ideal candidates for remediation of these salt affected soil and lands. Microalgae can be deployed for bioremediation at sites with highly variable salinities, without significant effects on end-product potential.

 

Speaker
Biography:

I have 22 years of experience in teaching, research and extension in the field of Fisheries Resources and Management. The job involves principally, teaching the undergraduate (B.F.Sc) and postgraduate (M.F.Sc and Ph.D.) students, in Fisheries Resources and Management courses covering Fish population dynamics and stock assessment, Taxonomy of finfish and shellfish, Biodiversity of finfish and shellfish, Marine fishery resources, Anatomy, Physiology of finfish and shellfish, Biology of fish, Tropical fish stock assessment, Applications of fisheries models in stock assessment and Modern techniques in ichthyotaxonomy. Besides the job includes research work of applied nature related to fisheries resources, their abundance, distribution, assessment and management. In addition, an effective extension work in fisheries is undertaken as a tool of taking the message from lab to land. Popular articles, interviews through local electronic media etc are also undertaken for the benefit of fisherfolk.

Abstract:

Fishes of the family Priacanthidae, popularly called big eye or bulls eye. Priacanthus hamrur is an important deep-water inhabitant of great commercial value. High percentage of landings of Priancanthids used as raw material for surimi, sausage and other fishery by-products. Presently, it has great demand in Singapore Thailand, Taiwan, Hong Kong and other countries. For the maturation studies samples were collected from commercial landing centre, Mangalore. Studies on reproductive biology showed that Priacanthus hamrur spawns twice in a year, the spawning season extending from March to May and October to November. Based on the percentage occurrence of mature fishes in various size group it was inferred that male attained maturity at smaller size than female. This study will enable us to understand the spawning periodicity, cyclic morphological changes in male, female gonads and also it helps to improve stock size by enforcing fishing ban in particular season by assessing spawning periodicity.

Speaker
Biography:

Dr Rajanna K.B. is an Assistant Professor at Fisheries Research and Information Centre (Inland), Hebbal, nataka Veterinary, Animal and Fisheries Sciences University. He has 8 years of service in the field of fisheries and aquaculture. Besides research, he is also involved in imparting training to the scientific and farming communities.

 

Abstract:

The ‘fringed - lipped’ peninsula carp Labeo fimbriatus (Bloch) is a potential and an abundant fish species of rivers and reservoirs of peninsular India. It contributes a part of inland fish production and also plays a role in rural economy in major carp deficient regions of India. The fish is locally called as “Kemmeenu” in Karnataka. Month wise samples were collected from the Vanivilasa Sagar Reservoir fish landing centre and fishing villages around the reservoir. For this study, a total of 1,126 specimens were examined during March, 2013 – February, 2014. Studies on the reproductive biology showed the occurrence of ripe gonads more during October, November, December and January. Thus it may be concluded that spawning season coinciding with monsoon season and the size at maturity was found to be 36 and 37 cm total length (M and F). Fecundity ranged from 63,897 to 5,10,824 with an average of 2,43,304 eggs and the predominance of female was reported. The pooled sex ratio (M: F) was found to be 1:1.09. This study will throw light on reproductive biology of fish for captive brood stock development, breeding and rearing of Labeo fimbriatus. Since this fish is commercial important the study would help to take up hatchery production.

  • Aquatic Resources & Environment Management

Session Introduction

M. Golam Mortuza

Department of Zoology, Faculty of Life and Earth Science, Rajshahi University, Rajshahi 6205, Bangladesh

Title: The Effect of Sewage Discharge on the Fish Faunal Diversity of Wadi Hanifah, Riyadh, Saudi Arabia
Speaker
Biography:

 M. Golam Mortuza is currently working as a Professor at University of Rajshahi, Bangladesh. He received his BSc and MSc in 1987 and 1988 respectively from University of Rajshahi, Bangladesh. He did his Doctor of Science (DSc) degree from Hiroshima University, Japan in 2005. He worked at Pusan National University as Postdoctoral Researcher in 2008-2009. During his postdoctoral research, his work has been patent registered in the Korean Intellectual Property Office.  In 2011, Dr. Mortuza accepted a Professor position at King Saud University, Riyadh, Saudi Arabia, where he was working on Environmental pollution and health risk assessment using fish. His research tropic addresses aquatic pollution and risk assessment, fish ecology, behaviour and systematic study of fish, floodplain fisheries. Dr. Mortuza also associate Research member with two other foreign University. Mortuza has over 50 refereed and ISI publications. Dr. Mortuza has also published a book chapter with other renounced authors in “Surface water photochemistry” Royal Society of Chemistry, London, 2015.

Abstract:

Effluent discharges from industries and municipal sewage from the city of Riyadh, Saudi Arabia and adjacent areas are partially carried out through a stream channel known as Wadi Hanifah. At present the main sources of water are sewage effluent, agricultural runoff, pumped shallow ground water from the city of Riyadh and seasonal rainwater. Monthly samples were collected from three sampling area (Namar, Al-Azizia and Al-Hair) from Wadi Hanifah during September 2014 to August 2015. Results indicated that the industrial and municipal effluent impacted negatively on water quality and consequently the fish community structure. A total of 17 fish species were recorded throughout the year with the lowest number of 11 species recorded in the most impacted Al-Azizia area. Species richness indices were highest at the Namar dam (recreation park area) and lowest at Al-Azizia, the most affected area, where vehicle mechanical industries are established and petrochemicals are damping through sewerage channels. Tilapia is dominating species in Wadi Hanifah especially at recreation park area, where fish species are culturing, while carp’s species are found throughout the channel. Due to high petrochemical pollution, dead fishes are usually found at Al-Azizia site. Low dissolved oxygen, pH, secchi depth and high electrical conductivity, total phosphorous, and total nitrogen, heavy metals were strongly associated with the effluent impacted sites and greatly influenced the fish community structure. This study recommends the use of fish as valuable biological indicators in aquatic environmental pollution assessment.

Speaker
Biography:

 M. Golam Mortuza is currently working as a Professor at University of Rajshahi, Bangladesh. He received his BSc and MSc in 1987 and 1988 respectively from University of Rajshahi, Bangladesh. He did his Doctor of Science (DSc) degree from Hiroshima University, Japan in 2005. He worked at Pusan National University as Postdoctoral Researcher in 2008-2009. During his postdoctoral research, his work has been patent registered in the Korean Intellectual Property Office.  In 2011, Dr. Mortuza accepted a Professor position at King Saud University, Riyadh, Saudi Arabia, where he was working on Environmental pollution and health risk assessment using fish. His research tropic addresses aquatic pollution and risk assessment, fish ecology, behaviour and systematic study of fish, floodplain fisheries. Dr. Mortuza also associate Research member with two other foreign University. Mortuza has over 50 refereed and ISI publications. Dr. Mortuza has also published a book chapter with other renounced authors in “Surface water photochemistry” Royal Society of Chemistry, London, 2015.

Abstract:

The study was carried out to assess surface water quality of Rajshahi City of Bangladesh. Based on surveys and chemical analyses, we performed a case study of the surface water collected from three different areas, in order to understand the sources of water pollution and the evolution of water quality in Rajshahi City, Bangladesh. Concentrations of major chemical elements in the surface water were related to the source of the agricultural, industrial and municipal sewerage. The Padma river bank close to the city side was more polluted by the domestic and sewerage system than that of opposite side.

Speaker
Biography:

Dr. Taoka has completed his PhD at the age of 28 years from the United Graduate School of Agricultural Sciences, Kagoshima University and postdoctoral studies from University of Miyazaki. He worked in a commercial fisheries company, Nippon Suisan Kaisha, Ltd. as a researcher and arrived at present post in University of Miyazaki at 2011. Recently, he has focused on the production of functional substances from marine microbes and the utilization in the field of aquaculture.

Abstract:

World aquaculture production has continued to increase. Fishmeal is used as protein sources for aqua-feeds. However, the price is drastically increased to around 170,000/ ton in 2013 during recent 10 years because of the decrease of fish resources, anchovy. This is serious problem for sustainable aquaculture. Therefore replacement of fishmeal to another resource is urgently needed. Several researchers reported the replacement of protein sources from fishmeal to plants. However, the complete replacement by plant proteins are not established   yet because plant sources contained some components that negatively affect the growth of the cultured fish due to the low digestibility. We focused on the “thraustochyrids” as probiotics to enhance the digestibility of plant sources.

Thraustochytrids are widely distributed in marine environment and have unique characteristics that accumulate large amount of lipids in cell bodies. Additionally, it is known that thrastochytrids produce various extracellualr enzymes including refractory components such as cellulose.

In this study, we researched the profile of extracellular enzymes from thraustochytrids and the tolerant ability in the gastrointestinal juices in vitro and in vivo to evaluate the potency as probiotics. Production of various extracellular enzymes, protease, amylase, lipase (tween 80 hydrolytic enzyme), cellulase and tannase were detected depending on the genus or species of thraustochytrids. The tolerance to artificial gastric juices was high in genus Aurantiochytrium and Ulkenia, and low in genus Oblongichytrium. In the case of in vivo test, Aurantiochytrim was recovered from the intestine of Japanese short-necked clam, Ruditapes philippinarum (“Asari” in Japanese). Additionally, we have isolated more than one hundred thraustochytrids from marine environment and are screening probiotic abilities to select adequate strain now. As next step, we are planning the rearing trial with aquaculture-major fish species and supply live thrautochytrids by oral administration

Speaker
Biography:

Abstract:

The present work was to optimize the purification conditions for soft-shelled turtle (Pelodiscus sinensis) calipash collagen (STCC) isolated by pepsin and to explore collagen physicochemical properties for potential biomaterial applications. Single-factor test and orthogonal method L9 (34) were employed with the STCC recovery yield as indicator. The optimum purification conditions were obtained when NaCl concentration, collagen concentration and purification time were 2 M, 8 g/L, and 24 h, respectively. Purified STCC were characterized by SDS-PAGE, UV scanning, FTIR, solubility, thermal behavior and amino acid analysis. The results showed that STCC contained high hydroxyproline content than that of other fishery skins, belonging to typical type I collagen in form of [α1(I)] 2α2(I). FTIR spectra of STCC were quite similar to other aquatic animals’ collagens. It has the lowest solubility at pH 6, and when NaCl concentration decreased from 2% to 6% (w/v), solubility dropped. The denaturation temperature (Td) and melting temperature (Tm) were 35.1 °C and 105.14 °C, respectively. Morphology of STCC depicted as regular and porous network structure by SEM. In general, the results suggested that turtle calipash can be exploited as alternatives to mammalian collagen and could also be used for biomedical applications as a potential new material.