Mr. Saberi Mawi, Ms. Amatul Samahah Md Ali, and Dr. Zainoddin Jamari are all working in the Fisheries Research Institute (FRI), under the Deparment of Fisheries, Malaysia. Both Dr. Zainoddin Jamari and Mr. Saberi Mawi have more than 15 years of experience in the shrimp research area. The project was carried out in the facilities of FRI. Mr. Mohd Afiq Razi Md Razi and Mr. Yeong Kun Yong are both working for Rentas Saga Sdn. Bhd., the company involved in this project. The PLs source was brought in by the company.
A joint project between Fisheries Research Institute (FRI), Dept. Fisheries Malaysia and a private company Rentas Saga Sdn. Bhd. was initiated to help enhance the local prawn industry activities. In this project we would like to compare the growth performance and survival of two different domesticated source of black tiger prawn (Penaeus monodon). The domesticated black tiger shrimp were from Asia Pacific family and from Madagascar family. Both specific pathogen free (SPF) stocks were obtained from local hatcheries. The grow-out performance of these stocks have been reportedly showed an improved growth. Due to that these SPF stocks are now widely used in India, Thailand, and Vietnam. A few farms in Malaysia have been using both domesticated SPF stocks. However, the growth performance and survival for these two stocks have not been well-documented locally. Thus, in this study the growth performance and survival rate of Black Tiger Prawn from both domesticated stocks in brackish water ponds were recorded and evaluated. The prawns were reared in six earthen ponds of the same size (0.2 ha) located in FRI Gelang Patah, Johor, Malaysia and were carried out within the same culture period. Three pond A1, A2 and A3 were stocked with domesticated postlarvae(PL)s from Asia Pacific family and another three, B1, B2 and B3 were stocked with domesticated PLs from Madagascar family. Stocking density in each pond was standardized at 60 PL/m2. Water quality parameters were monitored regularly. Commercial prawn feed were given to the cultured prawns and the ponds were harvested after 120 days of culture. The average final weight of black tiger prawn cultured in pond A1, A2 and A3 is 37.0 ±1.1 g and in pond B1, B2, and B3 is 31.2 ±1.3 g. Average specific growth rate (SGR) for pond A1, A2 and A3 is 2.78 ±0.07 %/day while for pond B1, B2 and B3 is 2.27 ±0.03 %/day. The average survival rate of PLs were 86.73 ±6.30% and 83.13 ±5.01% for Asia Pacific family and for Madagascar family respectively. Based on the SGR and survival rate data of both PLs, PLs from Asia Pacific family showed better performance. However, this study only look at the grow-out phase of the two different source of PLs, further studies need to be carried out to see the differences in terms of growth and survival at the prematuration phase and maturation phase.
Che Zulkifli Che Ismail served as a research officer at Fisheries Research Institute of Malaysia since 1995 till present. He has expertise on marine finfish breeding including broodstocks management, larval rearing, nursing of fry and grow out. The past project that he already done was breeding of Cobia, Asian Sea bass, Tiger Grouper and Red Snappers. Beside the marine finfish, he also has an experience on breeding of tropical abalone, Green Muscle and Giant Freshwater Prawn. Beside the aquaculture field, he also has an experience on ecological study especially on the pollution of pesticide in the water, sediment and aquatic animal. Currently, he is studying at PhD level at University Malaysia Terengganu, Kuala Terengganu, Malaysia. His current study is the cryopreservation of Giant Grouper spermatozoa in order to produce the hybrid grouper fry.
Protein is a macromolecule that plays an important role in the living organism. The role of protein is catalysing the metabolic reaction, DNA replication, responding to stimuli and shipping the molecule from point to point. The level of protein in spermatozoa is very important because it’s contained the DNA information which can be transmitted from paternal to the next generation. The study was conducted to determine the level of total protein in cryopreserved semen and in spermatozoa of giant grouper ((Epinephelus lanceolatus) which preserved in dry ice (-79o Celsius) and shipped with Styrofoam box and dry ice as a refrigerant. The purpose of shipping is to use the cryopreserved spermatozoa in artificial insemination to produce hybrid grouper. The level of total protein also determined after the frozen semen are immersed back to liquid nitrogen after 24 hours and 48 hours in dry ice. The treatment in this study was 24 hours in dry ice, immersed back to liquid nitrogen after 24 hours in dry ice, 48 hours in dry ice, immersed back to liquid nitrogen after 48 hours in dry ice and 72 hours in dry ice. The other factor in the experiment is the container for the semen are loaded, which is straw and cryotube. The positive control for the experiment was the post thaw semen from liquid nitrogen and negative control for the experiment was fresh semen. The experiment also compared the level of total protein in semen before and after adding with extender and cryoprotechtant and also before and after cryopreservation procedure. The level of protein is determined using Bradford method. The result of the study shows there are no significant differences (P< 0.05) on the total protein from the semen and spermatozoa were loaded with straw and cryotube. There are significant differences (P< 0.05) on the level total protein in semen before and after adding with extender and cryoprotechtant. However, there no significant difference (P> 0.05) on the level of total protein before and after adding with the extender and cryoprotechtant in spermatozoa. There are no significant differences (P> 0.05) on the level of total protein in semen for all treatment and control. Thus, preservation and shipping the cryopreserved semen does not affect the level of total protein weather in semen or spermatozoa.