Nihon University, Japan.
Ryuichiro Miyazawa studies about T cell maturation and autoimmunity using zebrafish and ginbuna crucian carp models. Generally, zebrafish has been used as a model for embryogenesis. However, in these days, zebrafish is being used as a model of immune diseases. Lacking tools such as CD4 and CD8 mAb makes us difficult to comprehend the immune responses. In order to establish the immunological tools for zebrafish, he tries to apply anti-ginbuna CD4-1 and CD8α mAbs to zebrafish. He confirmed that anti-ginbuna mAbs react with zebrafish lymphocytes through gene expression analysis and dual staining with anti-hZAP-70 mAb.