Rahim Peyghan
Professor, Shahid Chamran University, Iran
Title: Cloning, expression and immunogenicity analysis of protective effect of recombinant protein (OmpTS) of Aeromanas hydrophila in Common carp, Cyprinus carpio.
Biography
Biography: Rahim Peyghan
Abstract
This study deals with the details of cloning, expression of recombinant ompTS of Aeromonas hydrophila and studied the immunogenicity of this protein in common carp. Escherichia coli strains DH5α and BL21 (Pasture institute, Iran) were used for cloning and expression of ompTS. For vaccination and challenge study, 270 fish were maintained under laboratory conditions. Blood sample was taken from fish of each group and assayed by enzyme linked immunosorbent assey (ELISA). The LD50 data revealed that LD50 value of Aeromonas hydrophila in this study was about 108.5cfu. We isolated A. hydrophila in kidney tissue of fish, indicating that the death was directly related to A. hydrophila. Significantly higher mortalities occurred in control groups (PBS, PBS+adjuvant) (76.66%, 56.66%) compared with fish vaccinated with recombinant OmpTS protein purified with NI-NTA column with(13.33%) and without adjuant (16.66%), and electroeluted method respectively (13.33%) and (23.33%), with and without adjuvantrespectively (PË‚0.05) (Figure 10 and 11). There was no significant differences between fish mortalities vaccinated with recombinant protein in both method of purification, with and without adjuvant (P≥0.05). The RPS value of fish vaccinated with NI-NTA OmpTS, NI-NTA OmpTS plus adjuvant, OmpTSelectroeluted and OmpTSelectroeluted plus adjuvant were 78.26%, 82.61%, 69.57 and 78.26% respectively. There was a significant differences between the RPS of fish vaccinated with recombinant protein in both method of purification, with and without adjuvant and control group (PË‚0.05).