A Chandrasekhara Rao
Asst.Professor, Sri Venkateswara Veterinary University, India
Title: Ultrastructural changes in the otocytes, hepatocytes and variations in the expression of yolk protein vitellogenin with the maturation of gonads in the Epinephelus diacanthus ( Valenciennes)
Biography
Biography: A Chandrasekhara Rao
Abstract
Ultrastructural changes in the oocytes and hepatocytes in the female Epinephelus diacanthus were studied with the progress of maturation. Transmission Electron microscopic (TEM) observations revealed cytological changes associated with ovarian development. Nucleolus number increased in the Peri Nucleolus stage, which is an indirect indication of increase in protein synthesis with the onset of oogenesis. Zonation of yolk sphere and presence of microvilli in the Zona radiata were observed in mature oocytes. In comparision to the immature phase, mature/ripe stage hepatocytes showed greater development of both endoplasmic reticulum and increased density of mitochondria in the cytoplasm which is an evidence of progress in vitellogenin syntshesis. Yolk protein vitellogenin was partially characterized employing polyacrilamide gel electrophoresis (PAGE). Vitellogenin level of expression was increased with the maturation of gonads.