2^nd Global Summit on Aquaculture & Fisheries July 11-13, 2016 Kuala Lumpur, Malaysia

Vlastimil Stejskal has completed his Ph.D. at the age of 28 years at Faculty of Fisheries and Protection of Waters of University of South Bohemia in Ceske Budejovice. He is the lab leader of Laboratory of controlled reproduction and intensive fish culture. He has published or co-authored 19 papers in reputed journals with IF.

Recently, the intensive aquaculture (monoculture) of both species Eurasian perch (Perca fluviatilis) and pikeperch (Sander lucioperca) mainly in recirculation aquaculture systems is raised in Europe. The aim of the present study was to investigate the effects of monoculture and three different duoculture groups of these two percids on growth performance, survival and feed utilization. Five months-old pond-reared and habituated perch (Pe) and pikeperch (Sa) with initial weight of 11.3±2.2 and 11.4±2.1g, respectively were used in growth trial lasted 82 days. Fifteen tanks (70 L) of special construction allowed separation and counting of uneaten food were used and the fish were equally divided to 5 groups with tree replicates. Each tank contained 80 fish. Duoculture groups contained 75% of perch and 25% of pikeperch (75Pe25Sa), 50% of perch and 50% of pikeperch (50Pe50Sa) and 25% of perch and 75% of pikeperch (25Pe75Sa) were established. Moreover, monoculture of perch (100Pe) and pikeperch (100Sa) were tested as two controls. Significantly higher (p < 0.05) final body weight was found for pikeperch in group 25Pe75Sa compare to monoculture group (100Ca). Moreover, significantly lower final body weight was found for perch in group 75Pe25Sa compare to monoculture group (100Pe). Intraspecific comparison showed significantly better growth potential for pikeperch.There were significant difference in FCR between groups with better food utilization in group 25Pe75Sa followed by monoculture of pikeperch. Poorest FCR was found in duoculture combinations with majority of perch. Especially, comparison of monocultures presented better food utilization for pikeperch than perch. There was no significant difference in mortality between groups.

Raquel Silveira Coffigny has completed PhD in Biological Research Center from Baja California Sur, Mexico. She conducted research in: linking variations blood parameters with pathologies and environmental changes; diagnosis of viral, bacterial and parasitic diseases interest in aquaculture farming, and the use of plant extracts for the control of diseases. She has heading projects: Implementation of National Aquatic Health System, Management of invasive aquatics species for commercial exploitation. She is Deputy Director of Fisheries Research Center of Cuba for SeaFood Safety and Aquaculture Health. She is member of the editorial committee of the Cuban Journal Fisheries Research. She published more than 30 papers in indexed journals.

The animal aquatic health surveillance programs have become a requirement for the effective management of sustainable aquaculture. In 2005 was implemented in Cuba, a program based on Monitoring for pathogen and disease detection, as well as the implementation of biosecurity principles, systematic preparation of stakeholders, implementation of modern diagnostic techniques, prevention and control, use and application of effective medicines, environmentally safe. In freshwater fish (tilapia, carp and catfish) surveillance was established for detection of bacterial hemorrhagic septicemia and protozoa and helminth parasites. In marine fishes Lutjanus analis, Centropomus undecimalis and Rachycentrum canadum surveillance was established for detection Viral Encephalopathy and Retinopathy (VER), Vibrio and other pathogenic bacteria in cobia and protozoa and helminth parasites. Shrimp surveillance was established for listed OIE virus. Sampling was random and the sample size was calculated for 95 % confidence in detecting a single infection with 5 % prevalence. The diagnosis was made by conventional and molecular biology techniques. No detected none of the viral diseases OIE listed for L. vannamei. New pathogens were detected, three viruses in shrimp Litopenaeus schmitti, one bacterial disease in L. vannamei, were identified 23 new parasites and 5 new pathogenic bacteria in fishes. Drugs and antibiotics were tested as safe for use in the aquaculture; more than 15 natural medicines for the prevention and control of ectoparasites and bacteria were applied. The program has been effective and early detection of pathogens, has allowed the design of health management systems to reduce economic losses from disease outbreaks.

Graduate Degree in Social Sciences in 1983, specializing in Management, Sociology, Quality Management and Business by Graduate and Diploma courses. Leading consultancies in Colombia and Haiti on socioeconomic aspects in fishing communities of little economic development. He has participated in research projects linking social experience to aquaculture and fisheries research and participated in international projects related to the Protection and Conservation of Sea Turtles (WWF) and the present is a member of Project Development of sustainable aquaculture in Cuba, developed between the Fisheries Research Centre of Cuba and the Centre for Development Cooperation in Fisheries (CDCF) / Institute of Marine Research (IMR) Norway. Currently Deputy Director of Fisheries Research Center.

The participation of women in the economy, especially in the labor market and its potential contribution to development, we must analyze it as an integrated and comprehensive system approach. In Cuba, deep economic, political and social changes that have occurred have affected in a comprehensive manner the women´s rights. However in rural areas, "where there is a masculinization of the main economic activities, including fishing, are evident, there exist a contempt for women working in these types of tasks based on the idea that they are less productive than men ". It is now of high priority on the island to encourage full employment formula, which contribute to boosting growth rates of the national economy, and that in parallel guarantee a healthy ecosystem, to obtain healthy animals and to safeguard population health. Cuban aquaculture is of a great importance for the country, as it is the prospect of future development in the fishing industry. Aquaculture represents not only the supply of animal protein for the population, but also contributes to increasing exports. The present study analyze those zones where a high percentage of women are present and still do not have an employment relationship; those areas, with geographical conditions and potentiality for development of rural aquaculture, allow sustainable marine species farming to develop small-scale coops, providing to women to be inserted into a domestic form economy in social life. This will contribute to improve the livelihoods and food security.

Anuwat Uppanunchai has completed his PhD at the age of 40 years from Ubon Ratchathani University, Ubon Ratchathani, Thailand.He is the director of Lamphun Inland Fisheries Research and Development Center, Lamphun, Department of fisheries, Thailand. He has published more than 19 papers in reputed journals and has been serving as an editorial board member of repute.

Provision of adequate numbers of quality fish fry is often a key constraint on aquaculture development. The management of climate-related risks in hatchery and nursery management operations has not received much attention, but is likely to be a key element of successful adaptation to climate change in the aquaculture sector. This study explored the sensitivities and vulnerability of freshwater fish fry production in 15 government hatcheries across Northern Thailand to climate variability and evaluated the robustness of the proposed adaptation measures. This study found that hatcheries have to consider several factors when planning production, including: taking into account farmer demand; production capacity of the hatchery; availability of water resources; local climate and other area factors; and, individual species requirements. Nile tilapia is the most commonly cultured species of freshwater fish. Most fry production is done in the wet season, as cold spells and drought conditions disrupt hatchery production and reduce fish farm demand in the dry season. In the wet season, some hatcheries are impacted by floods. Using a set of scenarios to capture major uncertainties and variability in climate, this study suggests a couple of strategies that should help make hatchery operations more climate change resilient, in particular: improving hatchery operations and management to deal better with risks under current climate variability; improving monitoring and information systems so that emerging climate-related risks are known sooner and understood better; and, research and development on alternative species, breeding programs, improving water management and other features of hatchery operations.

Mohamed M. Said has completed his PhD at the age of 35 years from Ain Shams University and worked as Associate professor of Aquaculture at Suez University from 2011 until now. He is the Academic Coordinator of the marine fisheries technology program. He is responsible for designing and constructing the RAS research center, Suez University. He has published 4 papers in reputed journals and he is participating on establishing the first Egyptian intensive shrimp production system using bio-flock technology.

Line crossing analysis was conducted for growth traits (weight and length), condition factor (body weight (g) / length (cm)3) X 100 and for tolerance to some environmental extreme conditions (salinity and cold shock) using four lines of early larval stages of Nile tilapia Oreochromis niloticus; Giza (G), Fouky (F), Mariout (M), and Nag-Hamaady (N). Data were analyzed using two types of analysis, one involving the straight-breeding data and crosses and another using only the crosses. All line crossing effects (general combining ability (GCA), specific combining ability (SCA), maternal ability (MA), and sex linkage (SL) showed a highly significant effect (pï‚£0.0001 in most cases) on weight, length and condition factor. Both salinity tolerance and cold shock were highly significantly affected by GCA, SCA, and MA (pï‚£0.0001 in most cases) while SL affected salinity tolerance (pï‚£0.001) only. Overall heterosis for both weight and length was positive while negative estimates with no significant effect were noted for the heterosis in both salinity tolerance and cold shock. The results obtained from the two types of analysis were the same concerning the effects of GCA, SCA, and MA with some differences as regard to SL. Furthermore the ranks of the means are the same in both analyses. The general evaluation (overall mean plus all relevant crossing components) showed that FN cross showed superiority in body weight and length followed by NG where, NM and MN crosses were the least susceptible to cold shock among all crosses while NF cross attained the superiority in salinity tolerance.

Metekia Tamiru has completed his MSc from the Hebrew University of Jerusalem, The Robert H.Smith Faculty of Agriculture, Food and Environment, Rehovot, Israel. He has been lecturing and conducting research in the area of Fishery and Aquaculture at Jimma University, Ethiopia. He has three papers in press (to be published in reputable journals).

A few studies reported on differential susceptibility to the KOI Herpes virus disease among carp strains and in our lab, we have susceptible and resistant groups as well as a developed disease model. If and what differences in viral infection exist between resistant and susceptible is yet unknown. The objective of the study was to adopt and develop a protocol for quantifying KHV DNA in common carp tissue, and to use it for characterization of virus progression in infected fish from susceptible and resistant strains. In this study, phenol- and salt –based protocols were tested for extraction of DNA and suitability for KHV detection PCR. Salt-based extraction was better in terms of nucleic acid yield, quality and PCR product amplification. To test the viral infection dynamics, fish from resistance and susceptible families were exposed to the virus and tissues from three fish of each group were sampled at days, 0, 4 and 8 post infection. Regular PCR assay yielded strong and consistent amplification with fish-specific primers and no amplification with virus-specific primers. However, at TP8, different virus-specific primers gave consistent amplification in samples from all tissues. This result was corroborated by quantitative PCR, indicating that between days 4 and 8 the virus replicated considerably and spread in the fish body. This finding suggested that viral infection levels in the host tissues are still manageable until day 8 post infection, but susceptible fish are more prone to lose control over viral infection and hence die from this disease.

I am Dr Habib Zeighami Assistant Professor of Microbiology. I am 34 years old. I have published more than 24 papers in Microbiology journals and has been serving as an academic board member of Zanjan University of medical Scienses (Iran). I have 13 Msc of Food Microbiology student and we studied foodborne pathogens.

Food safety has emerged as an important global issue with international trade and public health implications. Bacterial pathogens as Vibrio parahaemolyticus recognized as an important cause of foodborne diseases related to the consumption of raw, undercooked or mishandled seafoods worldwide. A total of 100 individual Fish samples were collected from fish outlets in Zanjan, Iran and investigated for the presence of potentially pathogenic strains of V. parahaemolyticus. The fish samples were immediately homogenized and cultured on TCBS agar and subjected for confirmatory biochemical tests. Polymerase chain reaction (PCR) was performed for detection of total and pathogenic V. parahaemolyticus by amplification of vp-toxR, tdh and trh genes. The conventional method indicated that 14% of samples were positive for V. parahaemolyticus. However, PCR verified that only 8% of fish samples were positive for V. parahaemolyticus. Of the 100 fish samples in our study, only 1% tdh and 3% trh positive strains were identified. Detection of tdh and/ or trh positive V. parahaemolyticus in zanjan marketed in Zanjan, Iran shows a probable risk for public health. Therefore, the reliable molecular methods for monitoring of potentially pathogenic V. parahaemolyticus are strongly recommended for the routine seafood examination

I am Dr Fakhri Haghi Assistant Professor of Microbiology. I am 34 years old. I have published more than 22 papers in Microbiology journals and has been serving as an academic board member of Zanjan University of medical Scienses (Iran). I have 12 Msc of Food Microbiology student and we studied foodborne pathogens

Food safety has emerged as an important global issue with international trade and public health implications. Bacterial pathogens as Vibrio parahaemolyticus recognized as an important cause of foodborne diseases related to the consumption of raw, undercooked or mishandled seafoods worldwide. A total of 70 individual wild shrimp samples were collected from shrimp retail outlets in Zanjan, Iran and investigated for the presence of potentially pathogenic strains of V. parahaemolyticus. The shrim samples were immediately homogenized and cultured on TCBS agar and subjected for confirmatory biochemical tests. Polymerase chain reaction (PCR) was performed for detection of total and pathogenic V. parahaemolyticus by amplification of vp-toxR, tdh and trh genes. The conventional method indicated that 16 (22.8%) of samples were positive for V. parahaemolyticus. However, PCR verified that only 12 (17.1%) of shrimp samples were positive for V. parahaemolyticus. Of the 70 shrimp samples in our study, only 2 (2.8%) tdh and 1 (1.4%) trh positive strains were identified. Detection of tdh and/ or trh positive V. parahaemolyticus in shrimp marketed in Zanjan, Iran shows a probable risk for public health. Therefore, the reliable molecular methods for monitoring of potentially pathogenic V. parahaemolyticus are strongly recommended for the routine seafood examination.