Day 3 :
- Track 8: Aquaculture Disease & Chemotherapeutics
Track 9: Women in Aquaculture and Fisheries
Track 11: Aquaculture in Asia-Pacific Countries
Track 12: Aquaculture Marketing and Business
1Australian Nuclear Science and Technology Organisation
Time : 09:30-09:55
Nunik Cokrowati has completed her Master at the age of 26 years from Gadjah Mada University. She is lecturer at aquaculture study program Mataram University. She has published more than 5 papers in local journals and 1 paper in international journal. She concern at seaweed cultivation research and plankton researh.
This research purposes are to increase the production of seaweed species Eucheuma or Kappaphycus spp. adaptive to changes in water conditions and to produce high quality of seaweed continuosly accepted by industry troughout the year. In order to achieve these objectives, suitability analysis of Eucheuma or Kappaphycus spp using different cultivation methods and the stimulation of the growth of Eucheuma or Kappaphycus spp by adding Sargassum aquifolium extract was carried out. The research method used was the application of an integrated action research and involvement of farmers who will implement the research purposes directly. The result shows that the combined use of Eucheuma or Kappaphycus spp. and method of cultivation significantly affects the absolute growth of Eucheuma or Kappaphycus spp. and content of carrageenan. The other results of this research shows that the application of Sargassum aquifolium extract has no effect on the growth of Kappaphycus alvarezii. The conclusion of this research is the best growth of Eucheuma spinosum is found in cultivation using longline method, the best growth Kappaphycus striatum is found in cultivation using bottom-off method, and the best growth Kappaphycus alvarezii is found in cultivation using raft method. The application of Sargassum aquifolium extract does not have a significant effect on the growth of Kappaphycus alvarezii but can increase level of carrageenan content.
Suleyman Demirel University
Title: The effect on fatty acid content of rotifer (Brachionus plicatilis) of suplemented of L-Carnitine to algamac 3050, Olio ω-3
Time : 09:55-10:20
Sevgi Savas has completed her PhD at the age of 25 years from Egean University and postdoctoral studies from Aquaculture Department, Fisheries Faculty, Süleyman Demirel University. She is the dean of Fisheries Faculty.
In this study, it was determinated of the effect on fatty acid contens of rotifer (Brachionus plicatilis) of suplemented of L-carnitine to Algamac 3050, Olio ω-3 . S. parkle was used as feed in mass culture of B. plicatilis. Rotifer was applied feeding combined of commercial products suplemented with L-carnıtıne ; S. parkle, S. parkle + Olio ω-3, S. parkle.+ Olio ω-3 + L- karnitin, S. parkle + L-karnitin, S. parkle + Algamac 3050, S. parkle + Algamac 3050 + L-karnitin, S. parkle + Olio ω-3 + Algamac 3050, S. parkle + Olio ω-3 + Algamac 3050 + L-karnitin. Considerable differences were found in fatty acid composition of B. plicatilis fed with commercial enrichment diets. The highest EPA, DHA, PUFA were determinated enriched with S. parkle + Olio ω-3 + L-carnitine, S. parkle + Algamac 3050 + L-carnitine,S. parkle + Olio ω-3 + Algamac 3050 + L-carnitine in rotifer, respectively at 6 hour. The enrichment duration was found significant (P< 0,05) effect of PUFA content of rotifers.
Universiti Malaysia Terengganu
Time : 10:35-11:00
Dr Fazliyana has completed her PhD at the age of 27 from the University of Auckland, New Zealand. She is currently a lecturer at School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu. She has published widely and participated at many national and international conferences, and has been recognised with many awards and citations for her outstanding research. She was able to publish in top journals such as Food Chemistry, Food Hydrocolloids and Polymer Chemistry Journals. Her research interests include fish processing, utilisation of fishery by-products, extraction of collagen and its modification through chemical and enzymatic cross-linkings, and the physical properties of food proteins. Dr Fazliyana has been appointed as the external examiner for peer-reviewed journals, PhD and Masters theses. She has held teaching positions at the University of Auckland and Universiti Malaysia Terengganu. Dr Fazliyana has also been leading high-level programmes, and actively involved in many different organisations within the community that include organisations where she has volunteered, served on the board or other leadership role and participated in as a scientist. Dr Fazliyana is a member of New Zealand Institute of Food Science and Technology (NZIFST), International Food Technologist (IFT), New Zealand Institute of Chemistry (NZIC), and Malaysian Fisheries Society (MFS).
Gelatine is one of the most popular biopolymers widely used in food industries. It has been mainly derived from porcine skins and bovine bones. However, gelatine from marine sources has drawn extensive interest due to the requirements for Halal and Kosher ingredients in food product development and the rising concern of consumer about BSE in commercial mammalian gelatine products. Furthermore, the abundance of fishery by-products produced from keropok lekor industry has created the opportunity to extract the gelatine from fish sources. Therefore, the extraction of gelatine from several fish species was conducted and compared to the commercial mammalian gelatines. Fish gelatine exhibited relatively lower properties compared to the commercial ones. Therefore, modifications through chemical and enzymatic cross-linkings were conducted to improve its functional properties. The uncross-linked and cross-linked gelatine gels demonstrated distinctive differences in the molecular weight distribution, microstructure, gel strength, and degree of cross-linkings. The current findings have successfully proved that the modified fish gelatines can be an alternative to replace the mammalian gelatines. These improved functional properties could also create a significant demand for fish gelatine in food applications, in meeting the global requirements for Halal and Kosher markets as well as alleviating the waste disposal problem from the processing of keropok lekor.
Thailand Institute of Scientific and Technological Research
Time : 11:00-11:25
Narin Chansawang has completed her PhD in 2015 from University of Essex at England. She is a researcher of Bioscience department, Thailand Institute of Scientific and Technological Research. She is doing research about fresh water and marine algae.
A change in environmental conditions often leads to changes of physiology and biochemical composition of microalgae. Temperature is an important environmental factor regulating the growth of microalgae. In this study, the elemental and biochemical composition were measured in the marine haptophyte Emiliania huxleyi (clone CCMP 1516) in nutrient replete conditions. Triplicate cultures were incubated from 14oC to 22oC. Culture were illuminated under photon flux densities (PFD) 600 μmol photon m−2 s−1 on a 14L:10D cycle. The growth rate (GR) of E. huxleyi increased with temperature from 0.41 d-1 at 14oC to 1.12 d-1 at 22oC. Cell volume also varied with temperature. Cellular chlorophyll a, nitrogen, phosphorus, carbon and contents were also lower at 22oC than other temperatures. Protein, total amino acids from free and combined amino acid, and total pigments [mol accessory pigment (mol chl a)-1] were decreased with increasing temperature; however, the opposite response was observed in fatty acids. Myristic (C14:0) and oleic (C18:1) acid were predominant fatty acid approximately 18.1-22.9% total fatty acid. Eicosapentanoic acid (EPA, C20:5n3) and docosahexaenoic acid (DHA, C22:6n3) as polyunsaturated fatty acids (PUFAs) were found in E. huxleyi. The content of the highest DHA was 8.2 % total fatty acid at the low temperature while EPA content did not change (3-4 % total fatty acid) with temperature. The highest level of n-3 to n-6 ratio of the remaining fatty acids (~ 2) was achieved at 14oC of E. huxleyi which would make E. huxleyi suitable as a feed stock for aquaculture.
Title: Mixed infection of abdominal segment deformity disease (ASDD) and Enterocytozoon hepatopenaei (EHP) in cultured Litopeneaus vannamei : histopathological and bacteriological studies
Time : 11:25-11:50
Dr. janakiram P has obtained his Ph.D in Zoology from Andhra University , Visakhapatnam , India in the year 1999 and continued his post-doctoral studies in the same University. He is working as an Assistant Professor( Senior) in the division of fish pathology, Dept. of Marine Living Resources, Andhra University. He has published 16 research papers in National and International journals. He has been serving as one of the reviewers for the Indian Journal of Fisheries.
The diseased shrimp samples of cultured Litopenaeus vannamei showing symptoms of Abdominal Segment Deformity Disease ( ASDD ) with retarded growth were collected during the period 2013-2014 from East Godavari and Srikakulam Districts of Andhra Pradesh, India and subjected to histopathological and microbiological studies. Shrimp Tissues (viz. Gills, gut, muscle and hepatopancreas) were fixed in Davidson’s fluid and processed for histopathology. Gills have revealed the presence of excessive vacuolization. The innermost serosal layer of gut wall were eroded and sloughed-off into the lumen. The layers showed disintegration and also found the presence of encapsulated cysts bearing the spores of Enterocytozoon hepatopenaei(EHP). The muscle fibres were highly disintegrated , irregular, wavy and with more lacunae. In some regions, heavy haemocytic infiltration was also observed. In hepatopancreas, the tubules were completely degenerated and empty with severe haemocytic infiltration into the inter-tubular spaces. Chromatin-margination and nuclear hypertrophy were found in the tissue. Microsporidian cysts of Enterocytozoon hepatopenaei (EHP ) were found inside the B cells of the hepatopancreatic tubule epithelium as granular inclusions. PCR studies confirmed the presence of EHP. Haemolymph collected from the diseased samples have revealed the presence of Vibrio harveyi, V. alginolyticus and V. vulnificus as the associated Bacteria. Our study is the first report from India for the mixed infection of ASDD and EHP in cultured L. vannamei
SILO S.P.A Time : 11:50-12:15
Title: Species independent efficacy of a specific composition of Monoglycerides of short and medium chain fatty acids in preventing EMS and controlling Vibrio parahaemolyticus, Aeromonas spp., Streptococcus uberis, Flavobacterium spp., Yersinia Ruckery, Pseudomonas spp. and Bacillus Cereus affecting shrimps and other aquatic species.
Time : 11:50-12:15
Manuela Parini has a degree in Languages and Marketing. In 2000 she became member of the R&D Department of SILO SpA (Italy). From 2004 to 2007 she participated at the EU-project “Feeding Fat Safety” aimed at improving the safety level of fatty by-products and derivates to be used in commercial feeds. She published and participated as co-author in more than 10 studies and was invited as speaker at several conferences. In October 2015 she held a scientific speech at the conference of European Aquaculture Society in Rotterdam.
A specific compositions of 1-Monoglycerides of Short and Medium Chain Fatty Acids (from C2 to C12), available on the market under the commercial name SILOhealth 108, proved to exert a selective antibacterial action against pathogenic bacteria independently from the environmental pH, being antibacterial also at pH 7-8, that is in the conditions of the organs of aquatic species like gut, liver, hepatopancreas and other biological apparatuses. The MIC of the Monoglycerides composition at buffered pH 7 – 8 against Vibrio parahaemoliticus, Vibrio mimicus, Bacillus cereus, Aeromonas hydrophila, Yersinia ruckeri resulted to be 0.1%, against Flavobacteriun and Pseudomonas spp. 0.01% while Lactobacillus was not inhibited by the Monoglycerides composition. The composition has been tested in experimental conditions by the Public Animal Health Institute Bruno Ubertini (Italy) in juvenile sturgeon infected with 11 000 000 CFU of Aeromonas hydrophila: in the group treated with the composition the mortality was reduced by 80% (p < 0.001) and the SGR was increased by 13% (p < 0.01) compared to the control group. Applications in field proved that the composition is able to exert its actions independently from the specie. Penaeus Vannameis stocked in 3 ponds of 2 mu each (210 shrimps per square meter) in Zanjiang-China showed typical clinical signs of EMS: hepatopancreas, gut and faecal matter whitening. Vibrio Parahaemoliticus was isolated. The administration of the composition for 5 days decreased the mortality by 80% and restored normal growth. Above effects are repeatable independently from specie, being active in shellfish, freshwater and marine fish.
University of the Philippines Visayas
Title: Measuring the economic empowerment of women using an index: an application to small-scale fisheries and agriculture in sebaste, antique
Time : 12:15-12:40
Ritchie Ann is curretnly PhD student in University of the Philippines Visayas
This study measured the economic empowerment of women from small-scale fisheries and agriculture sector of Sebaste, Antique. There were a total of 199 respondents selected using stratified random sampling. The Five Domains of Empowerment (5DE) Index was used in measuring the economic empowerment of study participants. Through this composite index, it was determined how women scored in the five domains of empowerment, namely production, resources, income, leadership, and time. The result of the study shows that women fishers are more economically empowered than women farmers. The two sectors showed high disparity in their scores on input in productive decision; autonomy in production; ownership of assets; control over use of income; group member; speaking in public; workload; and leisure. Group member indicator contributed largely to the disempowered population in both sectors. Although income of women farmers is higher than that of women fishers, the latter are still economically empowered which suggests that economic empowerment is not dependent on income alone. The study recommends that fisheries and agriculture organization for women should be established so that their needs and concerns will be heard and addressed. It is further recommended that government projects focused on enhancing women empowerment should also give importance on other factors such as organization and leisure and not just income to totally promote of women empowerment. Further studies on measuring women’s empowerment using other methods should be pursued to provide more information on women’s well-being.
Title: Inhibitory activity of bio-active compounds isolated from anadara granosa in shrimp health management
Time : 12:40-01:05
Mr. Sunil kumar Duddu has obtained his M.Sc degree in Marine Biotechnology from Andhra University , Visakhapatnam , India in the year 2012 and continuing for Ph.D degree in the same University. He is currently working on the bioactive compounds of marine origin for shrimp health management. He has published 6 research papers in National and International journals.
Shrimp aquaculture production in the world is depressed mainly by diseases. Usage of antibiotics in aquaculture was banned as a precautionary measure to protect from bacterial resistance and bioaccumulation. In view of the above consequences, an alternate chemical or compound is essential to protect the stock. Hence, an intertidal mollusc i.e. Anadara granosa (Bivalve) has been selected for the isolation of bio active compounds. The crude extract was isolated from the muscle of A. granosa. and concentrated. It was tested against two pathogenic bacteria of the shrimp and fish viz. Vibrio harveyi and Staphylococcus aereus respectively by agar well diffusion method and maximum activity was shown against Vibrio harveyi by methanol and chloroform (9:1) extract. The crude extract was further subjected to contact bioautography guided screening to find out number of compounds showing antibacterial activity. Based on the results obtained in the above studies, the crude extract was also subjected to column chromatography studies to separate various fractions responsible for the antibacterial activity. Twelve fractions were obtained from the column chromatography and tested for their antibacterial activity. Out of twelve, five fractions have shown highest antibacterial activity and further run on column chromatography for purity. Each pure sub-fraction was subjected to NMR to observe the proton, C13 and H1-H1 correlation for the structure elucidation studies.